Composite

Part:BBa_K4940010

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Transcription unit of Prα-POL-his

This is a transcription unit in pET-28a, of gene Prα-POL-his tag. Transcription will be initiated through T7 promoter, and terminated by T7 terminator. Additionally, lac operator can control gene expression only possible when induced by IPTG, which promises biological safety.

Usage and Biology

More specific information can be found in BBa_K4940003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 140
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None